Rapid flow-cytometric susceptibility testing of Candida species

نویسندگان
چکیده

منابع مشابه

Rapid flow-cytometric susceptibility testing of Candida species.

OBJECTIVES To develop a rapid flow-cytometric antifungal susceptibility test and to compare results with the standard methods. METHODS Reference and laboratory strains of Candida were tested for susceptibility to fluconazole and echinocandin by fluorescent flow cytometry using Acridine Orange as indicator of viability. Flow cytometry results were compared with MICs as determined by macrodilut...

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Antifungal susceptibility testing of Candida species by flow cytometry.

The feasibility of flow cytometric antifungal susceptibility testing has been studied using the fluorescent anionic membrane potential probe, bis-(1,3-dibutylbarbituric acid) trimethine oxonol [DiBAC4(3)]. The in vitro antifungal susceptibility testing of amphotericin B was performed on 8 Candida isolates from clinical specimens and 2 ATCC strains by flow cytometry with the results compared to ...

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Molecular identification and in-vitro antifungal susceptibility testing of Candida species isolated from patients with onychomycosis

Background and Purpose: Candida species are the most opportunistic fungi affecting the nails and resulting in onychomycosis. In this study, we identified and evaluated in-vitro susceptibility of the recovered isolates against fluconazole (FLC), voriconazole (VRC), and clotrimazole (CLT) using the Clinical and Laboratory Standards Institute (CLSI) M27-A3 document. Materials and Method...

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Identification and Antifungal Susceptibility Testing of Candida species isolated from Bronchoalveolar Lavage sampl

The frequency of fungal infections in immunocompromised patients, particularly by Candida species, has increased in recent years. Colonization by Candida species in respiratory tract in susceptible hosts may play an important role to precede disseminated candidiasis. This study was designed to identify Candida species from bronchoalveolar lavage (BAL) samples and determination of antifungal sus...

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Rapid Cytometric Antibiotic Susceptibility Testing Utilizing Adaptive Multidimensional Statistical Metrics

Flow cytometry holds promise to accelerate antibiotic susceptibility determinations; however, without robust multidimensional statistical analysis, general discrimination criteria have remained elusive. In this study, a new statistical method, probability binning signature quadratic form (PB-sQF), was developed and applied to analyze flow cytometric data of bacterial responses to antibiotic exp...

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ژورنال

عنوان ژورنال: Journal of Antimicrobial Chemotherapy

سال: 2005

ISSN: 1460-2091,0305-7453

DOI: 10.1093/jac/dkh492